Journal: Cell Proliferation

Article Title: Cyclic mechanical stress modulates neurotrophic and myelinating gene expression of Schwann cells

doi: 10.1111/cpr.12151

Figure Lengend Snippet: Immunofluorescent staining of Schwann cell marker S100 in RSC96 cells. All cells were stained homogenously by green fluorescent dye conjugated with antibody. (a) Nuclei stained with DAPI. (b) S100 protein stained green. (c) Merged image. Scale bar = 50 μm.

Article Snippet: Rat Schwann cell line RSC96 (American Type Culture Collection, Manassas, VA, USA) was used in the present study.

Techniques: Staining, Marker

Journal: Cell Proliferation

Article Title: Cyclic mechanical stress modulates neurotrophic and myelinating gene expression of Schwann cells

doi: 10.1111/cpr.12151

Figure Lengend Snippet: Brain‐derived neurotrophic factor ( BDNF ) expression and secretion by RSC96 cells under compressive and tensile stresses for different durations. Compressive stress down‐regulated expression and secretion of BDNF in a time‐dependent manner with a rebound at 24 h (a, c), while tensile stress promoted BDNF expression and secretion with mRNA peaking at 1 h, and secreted protein peaking at 24 h (b, d). *P‐value lower than 0.05.

Article Snippet: Rat Schwann cell line RSC96 (American Type Culture Collection, Manassas, VA, USA) was used in the present study.

Techniques: Derivative Assay, Expressing

Journal: Cell Proliferation

Article Title: Cyclic mechanical stress modulates neurotrophic and myelinating gene expression of Schwann cells

doi: 10.1111/cpr.12151

Figure Lengend Snippet: Expression of myelin‐related genes Sox10 , Krox20 and NRG1 by RSC96 cells under compressive and tensile stresses, for different durations. Both compressive and tensile stresses significantly down‐regulated Sox10, Krox20 and NRG1 at all time points. *P‐value lower than 0.05.

Article Snippet: Rat Schwann cell line RSC96 (American Type Culture Collection, Manassas, VA, USA) was used in the present study.

Techniques: Expressing

Journal: Cell Proliferation

Article Title: Cyclic mechanical stress modulates neurotrophic and myelinating gene expression of Schwann cells

doi: 10.1111/cpr.12151

Figure Lengend Snippet: Expression of neural adhesion molecules NCAM and N‐cadherin by RSC96 cells under compressive and tensile stresses, for different durations. Compressive and tensile stresses significantly down‐regulated NCAM at all time points, while expression of N‐cadherin was not affected by either type of mechanical stress. *P‐value lower than 0.05.

Article Snippet: Rat Schwann cell line RSC96 (American Type Culture Collection, Manassas, VA, USA) was used in the present study.

Techniques: Expressing

Journal: bioRxiv

Article Title: Stage-specific extracellular vesicle cargo from Schwann cells orchestrates peripheral nerve regeneration

doi: 10.1101/2025.09.08.674933

Figure Lengend Snippet: (A) Schematic of the in vitro differentiation model used to induce Schwann cells (SCs) to induce myelination, followed by reversion to a repair phenotype. Rat primary SCs were treated with 1 mM dbcAMP in rat SC basal media for 72 h to induce differentiation into myelinating SCs, followed by dbcAMP withdrawal to reprogram into repair SCs. (B) Phase contrast and (C) phalloidin-stained super-resolution fluorescence imaging illustrates morphological differences and cytoskeletal organization in immature, myelinating, and repair SCs. Scale bars: 20 µm and 10 µm, respectively. (D) Immunofluorescence staining for SC-specific markers p75 NTR , SOX2, c-Jun, and KROX20 shows distinct molecular profiles for differentiation stages. Immature SCs express high p75 NTR , c-Jun, and Sox2; myelinating SCs express KROX20; repair SCs re-express immature proliferative markers. Scale bars: 100 µm. (E) Western blot analysis confirms different expression patterns of SC markers across distinct differentiation stages (the full-length blot images were available in Supplementary Figure 1). (F) Western blot quantification shows downregulation of p75 NTR , c-Jun, and Sox2 as normalized to actin in myelinating SCs, with significant upregulation in repair SCs. KROX20 and MBP were elevated in myelinating SCs. The data are presented as the mean ± SEM of three biological replicates. Statistical analysis was performed by using one-way ANOVA with Tukey’s multiple comparisons test: * p < 0.05, ** p < 0.005, and *** p < 0.001, ns, not significant.

Article Snippet: Primary Rat Schwann cells (RSC) (Catalog R842-05a) were purchased from CELL Applications (San Diego, CA, USA) and maintained according to the manufacturer’s protocol.

Techniques: In Vitro, Staining, Fluorescence, Imaging, Immunofluorescence, Western Blot, Expressing

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 1. Optical micro- scopic images of a Schwann cell sheet: (A) Actual size after detachment (1-cm scale); (B) 1 · microscopic image; (C) 63 · images of stained Schwann cell sheet; (C1) Phalloidin, (C2) DAPI, and (C3) merged. Schwann cells in the sheet structure were obvi- ously compacted and over- lapped together; (D) Z- stack images of Schwann cells and Schwann cell sheet stained with S-100 (shown in green). (D1) The thickness of confluent Schwann cells on a regu- lar culture plate was around 8–12 mm. (D2) The Schwann cell sheet after detachment was around 30- mm thick. The data sug- gested that Schwann cell sheets were multilayered. Color images available online at www.liebertpub .com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Staining

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 2. Microscopic images ( · 20) of pri- mary antibody S-100 stained on (A) Schwann cells seeded on a regular well plate; (B) Schwann cells on a thermoresponsive sub- strate surface. The samples were then incu- bated with Alexa Flour 488-conjugated secondary antibodies (shown in green). S-100 was highly expressed in the Schwann cell sheet, suggesting that thermoresponsive substrates in the study did not affect phe- notypic expression of Schwann cells. Color images available online at www.liebertpub .com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Staining, Expressing

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 4. Polymerase chain reaction (PCR) analysis of Schwann cell sheets and Schwann cells on a regular culture plate as the control group. Both samples were cultured for 1 day before extracting RNA. Primers of nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) were tested with the RNA by the reverse transcription–PCR assay. Rat beta-actin was used as a loading control. The result indicated that the Schwann cell sheets produced both neu- rotrophins as Schwann cells in the control group.

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Polymerase Chain Reaction, Control, Cell Culture, Derivative Assay, Reverse Transcription, Produced

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 3. Live-Dead Reduced Biohazard Viability/Cytotoxicity kit images: (A) Live–Dead image of Schwann cells seeded on a tissue well plate; (B) Live–Dead image of a Schwann cell sheet after detachment. Both images showed a significantly higher number of live cells (fluorescent green) than dead cells (fluorescent red). This result indicated that the thermoresponsive substrates and incubation in 4C for 2 h to detach the cell sheet did not harm the cells. Color images available online at www.liebertpub.com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Incubation

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 5. Proliferation rate of individual Schwann cells on tissue culture plates on days 1–4 (blue) and those on the plates coated with the ther- moresponsive substrates (green): 100,000 cells were cultured on day 0, and the cell number was counted on days 1–4 by a hemocytome- ter. The cells were confluent enough to harvest as a cell sheet on day 4 after detach- ment. The result indicated that the proliferation rate of Schwann cells (green) was significantly lower than the one in the control group (blue) due to the impact of thermoresponsive substrates. Color images available online at www.liebertpub.com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Cell Culture, Control

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 7. Western blotting analysis that compared the cell– cell interaction proteins, Pan-cadherin, N-cadherin, Beta- catenin, E-cadherin, and Actin of individual Schwann cells (left) and Schwann cell sheet (right). Both samples were prepared from the cells that were cultured in the same period of time. The western blotting analysis revealed that the cadherins of the Schwann cell sheet after detachment were similar to those of individual Schwann cells. In other words, the process of generating cell sheets still maintained a high level of cadherins upregulated during the cell culture and important to many tissue engineering applications. Color images available online at www.liebertpub.com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Western Blot, Cell Culture

Journal: Tissue Engineering Part A

Article Title: Characterization of Schwann Cells in Self-Assembled Sheets from Thermoresponsive Substrates

doi: 10.1089/ten.tea.2012.0516

Figure Lengend Snippet: FIG. 8. (A) Schwann cells on nanofibers stained with DAPI/Phalloidin (0.10-mm scale); (B) a phase-contrast image of the Schwann cell sheet on nanofibers after 48 h ( · 10) and (C) · 40. The result showed that indi- vidual Schwann cells and those in the sheet form per- formed cell migration through nanofibers that would assist the cells to mi- grate to the target location if the cell sheet is placed for nerve tissue regeneration. Color images available online at www.liebertpub.com/tea

Article Snippet: Rat Schwann cells (ATCC; CRL 2941) were cultured in a medium containing a 1:1 mixture of Ham’s F12 medium (Gibco) and Dulbecco’s modified Eagle’s medium lowglucose (Cellgro) supplemented with an antibiotic solution (1% penicillin–streptomycin; Sigma) and 10% fetal bovine serum (Atlanta Biologicals).

Techniques: Staining, Migration